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KMID : 0895820140240020001
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 2 p.1 ~ p.13
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Effects of Cheunggihwadamhwan Extract on Lowering Lipid, Antioxidation and Production of Proinflammatory Cytokines in Rats Fed on High Fat Diet
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Lee Han
Park Won-Hyung
Cha Yun-Yeop
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Abstract
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Objectives : The purpose of this study was to investigate the effects of Cheunggihwa-damhwan (CGHDH) extract on lowering lipid, antioxidation and production of proin-flammatory cytokines in rats fed on high fat diet.
Methods : 40 Male Sprague-Dawley rats were fed on high fat diet for 8 weeks and 32 rats (above 400 g) were randomly divided into 4 groups (8 mice in each group) : control group, 100 mg/Kg CGHDH group, 200 mg/Kg CGHDH group, 300 mg/Kg CGHDH group. We fed a control group of rats a basal diet and administered normal saline(100 mg/kg, 1 time/1 day) for 4 weeks. And We fed each experimental group of rats basal diet and administered an extract of Cheunggihwadamhwan extracts (100 mg/kg, 200 mg/kg, 300 mg/kg, 1 time/ 1 day) for 4 weeks. At the end of the experiment, the rats were sacrificed to determine their chemical composition. We measured lipid in plasma and liver, concentration of proin-flammatory cytokines, antioxidative activity and gene expression. The gene expression level was investigated by the way of reverse transcription-polymerase chain reaction (RT-PCR).
Results : 1. Concentration of plasma FFA, plasma TG, plasma total cholesterol and plas-ma LDL-cholesterol showed a significant decrement in Cheunggihwadamhwan groups. However, concentration of plasma HDL-cholesterol showed a significant increment in 200, 300 mg/kg Cheunggihwadamhwan groups. 2. Concentration of liver total cholesterol and liver TG showed a significant decrement in Cheunggihwadamhwan groups. 3. Concentra-tion of plasma TBARS showed a significant decrement in all Cheunggihwadamhwangroups. Concentration of liver TBARS showed a significant decrement in 200, 300 mg/kg Cheunggihwadamhwan groups. Concentration of liver GSH-Px, SOD and CAT showed a tendency to decrease in all Cheunggihwadamhwan groups. 4. Concentration of plasma IL-1β, plasma IL-6, TNF-α and NO, showed a tendency to decrease in all Cheunggihwa-damhwan groups. Concentration of plasma IL-10 showed a tendency to increase in all Cheunggihwadamhwan groups. 5. In the analysis of reverse transcription-polymerase chain reaction (RT-PCR), the gene expression of Apo-B and Apo-E in the Cheunggihwa-damhwan groups showed a low expression than that of control group. The ratio of Apo-B expression per β-actin expression in the showed a significant decrement in all Cheunggih-wadamhwan groups. The ratio of Apo-E expression per β-actin expression in the showed a significant decrement in 300 mg/kg Cheunggihwadamhwan groups.
Conclusions : According to this study, the extract of Cheunggihwadamhwan showed a positive effect of lowering lipid, antioxidation and a control of producing proinflammatory cytokines.
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KEYWORD
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Cheunggihwadamhwan, Lowering Lipid, Antioxidation, Inflammatory
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